In "Fallen Idols" (Season 5, Episode 5), the Pagan God Leshii fights the Winchesters as Paris Hilton, Abraham Lincoln, and Gandhi -- but NOT Beyoncé.
In "The Usual Suspects" (Season 2, Episode 7), Sam and Dean's practice of picking the first motel in the yellow pages and signing in as "Jim Rockford" is revealed to be the method they use to find each other when they get separated. Jim Rockford is a fictional private investigator from the 1970s TV series, The Rockford Files.
Charlie Bradbury (played by Felicia Day) first crosses paths with Sam and Dean while working as an I.T. expert at Richard Roman Enterprises. She is initially reluctant to get involved in the supernatural world by helping the Winchesters, but decides to become a hunter herself by her fourth episode.
Bobby Singer was first introduced to the supernatural world when his wife Karen was possessed by a demon. He was forced to kill her and afterwards dedicated his life to hunting supernatural creatures.
Shortly after they were unknowingly released by Castiel, the leviathans infiltrated human society, with their leader murdering and impersonating the real Dick Roman.
In "What's Up, Tiger Mommy?", an angel named Samandriel takes the vessel of a young man named Alfie who works at Weiner Hut. He bids for the Word of God tablet, but loses.
Jo Harvelle died while saving Sam and Dean from a hellhound sent to kill them by the demon Meg Masters.
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