Basil Karlo was the original Clayface, an actor driven insane when he learns that a classic horror film he starred in is being remade without him. Matt Hagen, the second Clayface, is a treasure hunter transformed into a malleable clay-like form by a mysterious radioactive pool of protoplasm. The third Clayface, Preston Payne, is a S.T.A.R. Labs scientist suffering from hyperpituitarism.
Killer Moth is the first villain Batgirl encounters in Detective Comics #359 -- her debut story. While driving to a costume ball dressed as a female version of Batman, Barbara Gordon foils an attempt by Killer Moth to kidnap Bruce Wayne. Although Batman insists she give up crime-fighting because of her gender, Batgirl disregards his objections.